Difference between revisions of "Part:BBa K2457005"

(Characterization)
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descrever
 
descrever
  
 +
[[File:BBa K2457005 circuit.png]]
  
 
===Usage and Biology===
 
===Usage and Biology===
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===Design===
 
===Design===
  
[[File:BBa K2457005 circuit.png]]
 
  
 
Figure 1: BBa K2457005 circuit
 
Figure 1: BBa K2457005 circuit

Revision as of 00:45, 2 November 2017


Standardized pBAD_Cas9 device

descrever

BBa K2457005 circuit.png

Usage and Biology

Design

Figure 1: BBa K2457005 circuit

Characterization

Sequencing

BBa K2457005 electropherogram.png

Figure 2: Sequencing electropherogram from BBa_K2457005.

BBa K2457005 aligmnent.png

Figure 3: Alignment of the designed sequence and our final construction from BBa_K2457005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1024