Difference between revisions of "Part:BBa K2486010"

 
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<partinfo>BBa_K2486010 short</partinfo>
 
<partinfo>BBa_K2486010 short</partinfo>
  
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This module works as a reporter for lactate an tetracycline presence wen on a system that have both regulatory proteins
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This module works as a reporter for lactate an tetracycline presence. The circuit's key part is the PlldR_tetR promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K2486011 BBa_K2486011]), a part inspired by ETH_Zurich 2015 team [http://2015.igem.org/Team:ETH_Zurich/Part_Collection parts collection] of hybrid promoters for LacI and LldR. We adopted tetR operator in our promoter instead of lacI operator because of it's tighter regulation, reducing
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:28, 2 November 2017


PlldR_tetR reporter circuit (GFP)

This module works as a reporter for lactate an tetracycline presence. The circuit's key part is the PlldR_tetR promoter (BBa_K2486011), a part inspired by ETH_Zurich 2015 team [http://2015.igem.org/Team:ETH_Zurich/Part_Collection parts collection] of hybrid promoters for LacI and LldR. We adopted tetR operator in our promoter instead of lacI operator because of it's tighter regulation, reducing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 100
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 810