Difference between revisions of "Part:BBa K2355006"
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It can be used to distinguish between these two Bitis snakes and <i>Naja nigricollis </i>. | It can be used to distinguish between these two Bitis snakes and <i>Naja nigricollis </i>. | ||
| − | + | In the graf below you are able to see the cleavage by all three venoms. | |
<html> | <html> | ||
<figure> | <figure> | ||
<img src="https://static.igem.org/mediawiki/2017/d/dd/T--DTU-Denmark--peptidescreenB11.png" alt="Substrate screening data for B11" width="500">' | <img src="https://static.igem.org/mediawiki/2017/d/dd/T--DTU-Denmark--peptidescreenB11.png" alt="Substrate screening data for B11" width="500">' | ||
| − | <figcaption>Figure 1: | + | <figcaption>Figure 1: Substrate with sequence AAGVAPLS (well B11) incubated with the three different venoms.</figcaption> |
</figure> | </figure> | ||
</html> | </html> | ||
Latest revision as of 00:15, 2 November 2017
Cleavable peptide sequence from Human Neuromedin-B
This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is AAGVAPLS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Results of experiments
The peptides below, are cleaved by Bitis arietans and Bitis gabonica. It can be used to distinguish between these two Bitis snakes and Naja nigricollis .
In the graf below you are able to see the cleavage by all three venoms.
'