Difference between revisions of "Part:BBa K2381024"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2381024 short</partinfo> | <partinfo>BBa_K2381024 short</partinfo> | ||
− | + | <p> | |
− | + | It’s a gRNA sequence containing a promoter of <partinfo>BBa_J23119</partinfo>, 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold.</p> | |
+ | [[File:T--HZAU-China--gRNA_function.png|200px|thumb|left|Its function is proved by OD measurement after cotransformed with plasmid containing dCas9.We can observe tight repression on cell growth. ]] | ||
+ | [[File:T--HZAU-China--gRNA_qPCR.png|200px|thumb|left|Further investigation is conducted by qPCR and flow cytometric. Its effect is even better than rapamycin, a well-used chemical molecule for replication inhibition.]] | ||
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Revision as of 00:07, 2 November 2017
J23119-gRNA (tightly target at oriC to inhibit cell replication)
It’s a gRNA sequence containing a promoter of BBa_J23119, 20bp targeting at oriC and a full-length gRNA scaffold.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 47
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]