Difference between revisions of "Part:BBa K2355008"
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One out of three peptides that were specifically cleaved only by <i>Bitis arietans</i>.
 
One out of three peptides that were specifically cleaved only by <i>Bitis arietans</i>.
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              <img src="https://static.igem.org/mediawiki/2017/f/f5/T--DTU-Denmark--peptidescreenB17.png" alt="Substrate screening data for B17" width="500">
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              <figcaption>Figure 1: Substrate with sequences RQRYGKRS (well B17) incubated with the three different venoms.</figcaption>
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Revision as of 00:01, 2 November 2017


Cleavable peptide sequence from Human Pro-neuropeptide Y

This is a protein linker with a peptide sequence of RQRYGKRS. This peptide sequence has been proven experimentally to be cleaved by the venom of Bitis arietans. It is originally from Human Pro-neuropeptide Y, a pro-peptide that is implicated in the secretion of gonadotrophin-release hormone (GnRH)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Results of experiments

One out of three peptides that were specifically cleaved only by Bitis arietans.

Substrate screening data for B17
Figure 1: Substrate with sequences RQRYGKRS (well B17) incubated with the three different venoms.