Difference between revisions of "Part:BBa K2381023"

Revision as of 00:00, 2 November 2017

PcpcG2-gRNA (light-control cell replication inhibitor) It’s a gRNA sequence containing a light-induced promoter, 20bp targeting at oriC and a full-length gRNA scaffold. It's function is blocking the replication initiation.

Its function is proved by OD measurement.

References
1. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810. 2. Schmidl, S. R., Sheth, R. U., Wu, A., & Tabor, J. J. (2014). Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol, 3(11), 820-831.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 250
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2381023 short</partinfo>
+It’s a gRNA sequence containing a light-induced promoter, 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold.
+It's function is blocking the replication initiation.
+[[File:T--HZAU-China--light_gRNA.png|200px|thumb|left|Its function is proved by OD measurement.]]
-dfks
+<p>References<br/>
+. Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.
+. Schmidl, S. R., Sheth, R. U., Wu, A., & Tabor, J. J. (2014). Refactoring and optimization of light-switchable Escherichia coli two-component systems. ACS Synth Biol, 3(11), 820-831.
+</p>
 <!-- Add more about the biology of this part here