Difference between revisions of "Part:BBa K2355006"
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The peptides below, are cleaved by <i>Bitis arietans</i> and <i>Bitis gabonica</i>.  
 
The peptides below, are cleaved by <i>Bitis arietans</i> and <i>Bitis gabonica</i>.  
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They can be used to distinguish between these two Bitis snakes and <i>Naja nigricollis </i>.
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It can be used to distinguish between these two Bitis snakes and <i>Naja nigricollis </i>.
  
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    https://static.igem.org/mediawiki/2017/d/dd/T--DTU-Denmark--peptidescreenB11.png
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<figure>
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              <img src="https://static.igem.org/mediawiki/2017/d/dd/T--DTU-Denmark--peptidescreenB11.png" alt="Substrate screening data for B11" width="500">'
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<figcaption>Figure 1: Substrates with sequence AAGVAPLS (well B11) incubated with the three different venoms.</figcaption>
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                  </figure>
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Revision as of 23:56, 1 November 2017


Cleavable peptide sequence from Human Neuromedin-B

This is a linker consisting of a peptide sequence, which has been proven experimentally to be cleaved by the venom of Bitis gabonica and Bitis arietans. The specific peptide sequence of the linker is AAGVAPLS


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



Results of experiments

The peptides below, are cleaved by Bitis arietans and Bitis gabonica. It can be used to distinguish between these two Bitis snakes and Naja nigricollis .

Substrate screening data for B11'
Figure 1: Substrates with sequence AAGVAPLS (well B11) incubated with the three different venoms.