Difference between revisions of "Part:BBa K2381012"
Line 5: Line 5:
 
<p>
 
<p>
 
It’s a gRNA sequence containing 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.<br/>
 
It’s a gRNA sequence containing 20bp targeting at <i>oriC</i> and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.<br/>
−
The targeting sequence of this part is acquired from reference paper. Its function after J23119 promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement after cotransformed with plasmid containing dCas9.<br/>
+
The targeting sequence of this part is acquired from reference paper. Its function after J23119 promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement after cotransformed with plasmid containing dCas9.</p>
−
[[File:T--HZAU-China--gRNA_function.png|200px|thumb|left|alt text]]<br/>
+
[[File:T--HZAU-China--gRNA_function.png|200px|thumb|left|alt text]]
−
We can observe tight repression on cell growth. Further investigation is conducted by qPCR and flow cytometric.<br/>
+
<p> We can observe tight repression on cell growth. Further investigation is conducted by qPCR and flow cytometric.</p>
−
[[File:T--HZAU-China--gRNA_qPCR.png|200px|thumb|left|alt text]] <br/>
+
[[File:T--HZAU-China--gRNA_qPCR.png|200px|thumb|left|alt text]]  
−
Its function after light-induced promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement.<br/>
+
<p>Its function after light-induced promoter, <partinfo>BBa_K2381020</partinfo>, is proved by OD measurement.</p>
 
[[File:T--HZAU-China--light_gRNA.png|200px|thumb|left|alt text]]  
 
[[File:T--HZAU-China--light_gRNA.png|200px|thumb|left|alt text]]  
−
</p>
+
 
  
 
<h3>References</h3>
 
<h3>References</h3>

Revision as of 23:45, 1 November 2017


3'-OriC gRNA

It’s a gRNA sequence containing 20bp targeting at oriC and a full-length gRNA scaffold. The sequence is central to our project and is related with all the system built this year.
The targeting sequence of this part is acquired from reference paper. Its function after J23119 promoter, BBa_K2381020, is proved by OD measurement after cotransformed with plasmid containing dCas9.

alt text

We can observe tight repression on cell growth. Further investigation is conducted by qPCR and flow cytometric.

alt text

Its function after light-induced promoter, BBa_K2381020, is proved by OD measurement.

alt text


References

Wiktor, J., Lesterlin, C., Sherratt, D. J., & Dekker, C. (2016). CRISPR-mediated control of the bacterial initiation of replication. Nucleic Acids Res, 44(8), 3801-3810.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]