Difference between revisions of "Part:BBa I763010"
 
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<partinfo>BBa_I763010 short</partinfo>
 
<partinfo>BBa_I763010 short</partinfo>
  
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Promoter lambda - cI regulated - (R0051) regulates the expression of LacI reppression protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.  
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Promoter lambda - cI regulated - (R0051) regulates the expression of LacI repressor protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting plac-driven transcription.  
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This biobrick is necessary to repress pLac activity in absence of enough endogenous LacI.
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Latest revision as of 16:50, 26 October 2007

LacI repressor regulated by Plamda Promoter

Promoter lambda - cI regulated - (R0051) regulates the expression of LacI repressor protein with an LVA degradation tail. LacI binds to the pLac regulator BBa_R0010 and PLlac01 hybrid regulator BBa_R0011 and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting plac-driven transcription.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]