Difference between revisions of "Part:BBa K2258000"
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Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium. | Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 23:15, 1 November 2017
pL and Pleft Hybrid promoter
Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.
Usage and Biology
Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.
Reference
- PL Expression System : A Prokaryotic Expression System, Invitrogen manual
- Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]