Difference between revisions of "Part:BBa K2455003:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Creation
+
The biobrick was created by amplifying a modified version of the [https://www.thermofisher.com/order/catalog/product/K10201 pET102 vector] with the following set of primers, followed by blunt ligation:
  
 +
Forward primer: CGTCGCCGTCGCCGT-GAATGCGTGCGATCGCG
  
 +
Reverse primer: GCGACGGCGACGCAT-GCGTATGTATATCTCCTTCTTAAAG
 +
 +
For creation of a biobrick version the following set of primers was sub-sequently used:
 +
 +
Forward primer: ATCCGGAATTCGCGGCCGCTTCTAG-ATGCGTCGCCGTCGCCGTCGCCGTCGCCGTGAATGCGTGCGATC
 +
 +
Reverse primer: CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTA-ATGGTGATGGTGATGATGTAAG
  
 
===Source===
 
===Source===

Revision as of 23:11, 1 November 2017


Cell-Penetrating USER Cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick was created by amplifying a modified version of the pET102 vector with the following set of primers, followed by blunt ligation:

Forward primer: CGTCGCCGTCGCCGT-GAATGCGTGCGATCGCG

Reverse primer: GCGACGGCGACGCAT-GCGTATGTATATCTCCTTCTTAAAG

For creation of a biobrick version the following set of primers was sub-sequently used:

Forward primer: ATCCGGAATTCGCGGCCGCTTCTAG-ATGCGTCGCCGTCGCCGTCGCCGTCGCCGTGAATGCGTGCGATC

Reverse primer: CCAATGCATTGGTTCTGCAGCGGCCGCTACTAGTATTATTA-ATGGTGATGGTGATGATGTAAG

Source

Synthetic

References