Difference between revisions of "Part:BBa K2301000"
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| − | T4 holin is a protein isolated from the T4 bacteriophage that lyses a bacterial cell from the inside. It opens pores in the cell membrane and wall, allowing lysozymes to degrade it. The bacteria dies as it is exposed to the extracellular environment. | + | T4 holin is a protein isolated from the T4 bacteriophage that lyses a bacterial cell from the inside. It opens pores in the cell membrane and wall, allowing lysozymes to degrade it. The bacteria dies as it is exposed to the extracellular environment. We incorporated this part along with BBa_K2301001,BBa_K2301002 and BBa_K2301003 into a temperature controlled kill switch for bacteria. |
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Revision as of 23:08, 1 November 2017
T4 Holin optimized for E. coli expression
T4 holin is a protein isolated from the T4 bacteriophage that lyses a bacterial cell from the inside. It opens pores in the cell membrane and wall, allowing lysozymes to degrade it. The bacteria dies as it is exposed to the extracellular environment. We incorporated this part along with BBa_K2301001,BBa_K2301002 and BBa_K2301003 into a temperature controlled kill switch for bacteria.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]