Difference between revisions of "Part:BBa K2258000"
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Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.
 
Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.
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===Reference===
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1.PL Expression System : A Prokaryotic Expression System, Invitrogen manual
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2.Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU
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Revision as of 23:06, 1 November 2017


pL and Pleft Hybrid promoter

Acts as shuttle promoter, since pL from Lambda phage allows expression in Escherichia coli and Pleft from Mycobacterio phage allows expression in Mycobacterium.


Reference

1.PL Expression System : A Prokaryotic Expression System, Invitrogen manual 2.Transcriptional regulation of repressor synthesis in mycobacteriophage L5, Chadd E. Nesbit, Margaret E. Levin, Mary K. Donnelly-Wu and Graham F. HatfuU


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]