Difference between revisions of "Part:BBa K2450251"
(→Usage and Biology) |
|||
| Line 13: | Line 13: | ||
CFP is a variation on Green Fluorescent Protein. | CFP is a variation on Green Fluorescent Protein. | ||
| + | |||
| + | For more information on how this part was used in our project, please see our wiki: http://2017.igem.org/Team:Oxford | ||
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2450251 parameters</partinfo> | <partinfo>BBa_K2450251 parameters</partinfo> | ||
Latest revision as of 22:59, 1 November 2017
TetR CFP
A Tet Repressor tagged with CFP. TetR binds to the tet operator to prevent the transcription of a downstream gene. Repression is relieved by tetracycline.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
TetR functions as a homodimer to bind tet operator sequences and prevent the association of RNA polymerase with its promoter. Each TetR monomer has a DNA binding domain and a dimerisation domain. The DNA binding domain is a helix-turn-helix motif that associates with a tet operator sequence (TCCCTATCAGTGATAGAGA). Our complementary part, BBa_K2450301, contains 2 repeats of this sequence - one for each monomer.
CFP is a variation on Green Fluorescent Protein.
For more information on how this part was used in our project, please see our wiki: http://2017.igem.org/Team:Oxford