Difference between revisions of "Part:BBa K2292006"
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This is a composite of mazEF that is regulated by Arabinose. When arabinose is added, mazEF will be produced. And once arabinose supply is in short,  mazE ceased to be produced, it would be degraded by the protease ClpAP.
 
This is a composite of mazEF that is regulated by Arabinose. When arabinose is added, mazEF will be produced. And once arabinose supply is in short,  mazE ceased to be produced, it would be degraded by the protease ClpAP.
  
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<img src="https://static.igem.org/mediawiki/parts/4/4d/MazEF~truth_23.jpg"  style="display:block; margin:auto;">
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https://static.igem.org/mediawiki/parts/4/4d/MazEF~truth_23.jpg
  
  

Revision as of 22:49, 1 November 2017


MazEF kill switch

MazEF is a suicide system in Escherichia coli which consists of a stable toxin mazF and labile antitoxin mazE. MazF causes cell death by cleaving mRNA at a specific site. On the other hand, mazE plays a role of antitoxin which can antagonize the toxin mazF.

In general, continuous generation of MazE can prevent the situation of cell death by antagonizing mazF. However, once mazE ceased to be produced, it would be degraded by the protease ClpAP. Hence, the remaining toxin mazF will cause the cell death by cleaving mRNA.

This is a composite of mazEF that is regulated by Arabinose. When arabinose is added, mazEF will be produced. And once arabinose supply is in short, mazE ceased to be produced, it would be degraded by the protease ClpAP.

MazEF~truth_23.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]