Difference between revisions of "Part:BBa K2292003"
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FLAG-TAG is added after comE and c-Myc is added after comD
 
FLAG-TAG is added after comE and c-Myc is added after comD
  
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<!-- Add more about the biology of this part here
 
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Revision as of 22:47, 1 November 2017


comDE two components signal transduction system

This is a composite used for producing comD, comE, two essential protein for the CSP two components signal transduction system. Originated from Streptococcus mutans. ComD is a kinase and membrane-associated receptor. After comD binded to the quorum sensing molecule CSP, the comD-CSP complex will phosphorylate comE. Then, the phosphorylated comE can turn on the transcription of downstream gene. FLAG-TAG is added after comE and c-Myc is added after comD

TCSTS~23~.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 268
    Illegal XhoI site found at 123
    Illegal XhoI site found at 153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]