Difference between revisions of "Part:BBa I751501"

Revision as of 16:39, 26 October 2007

plux-cI hybrid promoter

sense TWO INPUTS, activation by AHL and repression by Lambda cI.

Usage and Biology

The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though Assay 0. The results are shown in Fig.3. The newly devised hybrid promoter is activated by AHL and repressed by LacI.
In the present project, this hybrid promoter has been tested on the BBa_I751100. (J54140del Pr)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Fig.3: The result of assay0
Only in the presence of AHL and in the absence of lacI, the lux lac hybrid promoter is activated significantly.

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-[[Image : Hybridgraph.JPG|thumb|300px| '''Fig.3: The result of assay0''' <br>Only in the presence of AHL and in the absence of lacI, the lux lac hybrid promoter is activated significantly.|right]]
-<br>
+The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though [[http://parts.mit.edu/igem07/index.php/Tokyo/Works/Assay0| Assay 0]]. The results are shown in Fig.3.
-The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though '''[[http://parts.mit.edu/igem07/index.php/Tokyo/Works/Assay0|Assay 0]]'''. The results are shown in Fig.3.
 '''The newly devised hybrid promoter is activated by AHL and repressed by LacI.'''
 <br>In the present project, this hybrid promoter has been tested on the [[Part:BBa_I751100|BBa_I751100. (J54140del Pr)]]
@@ Line 22: / Line 21: @@
 <partinfo>BBa_I751501 parameters</partinfo>
 <!-- -->
+<br>
+[[Image : Hybridgraph.JPG|thumb|300px| '''Fig.3: The result of assay0''' <br>Only in the presence of AHL and in the absence of lacI, the lux lac hybrid promoter is activated significantly.|right]]