Difference between revisions of "Part:BBa K2271002"

Latest revision as of 22:42, 1 November 2017

Pex11-mVenus with ScPAB1 promoter

This part contains the coding sequence for the peroxin Pex11p with medium strong promoter ScPAB1 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein Venus fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell.

Usage and Biology

Pex11 is a peroxin located in the inner surface of the peroxisomal membrane and is required for peroxisome biogenesis [ 1 ]. Erdmann and Blobel have shown that the deletion of the PEX11 gene in S. cerevisiae results in cells with fewer, larger peroxisomes, whereas overexpression results in cells with a higher quantity of smaller peroxisomes [2].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal suffix found in sequence at 2132
Illegal BglII site found at 701
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2271002 short</partinfo>
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+This part contains the coding sequence for the peroxin Pex11p with medium strong promoter ScPAB1 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein Venus fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+Pex11 is a peroxin located in the inner surface of the peroxisomal membrane and is required for peroxisome biogenesis [ 1 ]. Erdmann and Blobel have shown that the deletion of the PEX11 gene in S. cerevisiae results in cells with fewer, larger peroxisomes, whereas overexpression results in cells with a higher quantity of smaller peroxisomes [2].
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