Difference between revisions of "Part:BBa I751501"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | [[Image : Hybridgraph.JPG|thumb|300px| '''Fig.3: The result of assay0''' <br>Only in the presence of AHL and in the absence of lacI, the lux lac hybrid promoter is activated significantly.|right]]<br> | + | [[Image : Hybridgraph.JPG|thumb|300px| '''Fig.3: The result of assay0''' <br>Only in the presence of AHL and in the absence of lacI, the lux lac hybrid promoter is activated significantly.|right]] |
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The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though '''[[http://parts.mit.edu/igem07/index.php/Tokyo/Works/Assay0|Assay 0]]'''. The results are shown in Fig.3. | The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though '''[[http://parts.mit.edu/igem07/index.php/Tokyo/Works/Assay0|Assay 0]]'''. The results are shown in Fig.3. | ||
'''The newly devised hybrid promoter is activated by AHL and repressed by LacI.''' | '''The newly devised hybrid promoter is activated by AHL and repressed by LacI.''' | ||
Revision as of 16:37, 26 October 2007
plux-cI hybrid promoter
sense TWO INPUTS, activation by AHL and repression by Lambda cI.
Usage and Biology
The characteristics of this hybrid promoter (Lux-lac hybrid promoter) have been determined though Assay 0. The results are shown in Fig.3.
The newly devised hybrid promoter is activated by AHL and repressed by LacI.
In the present project, this hybrid promoter has been tested on the BBa_I751100. (J54140del Pr)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]