Difference between revisions of "Part:BBa K2382015:Design"
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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The usage of thioredoxin is to make FGD more soluble. However we don't want the two small protein to interrupt the folding of each other. That's why we add some linkers between them.
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Theoretically, thioredoxin (BBa_K2382004)could help protein folding in the cell, thus,we combined the FGD(BBa_K2382002) and thioredoxin together to make a new fusion protein. The two glycine between the linker and FGD are designed for a better flexibility.
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===Source===
 
===Source===

Latest revision as of 22:25, 1 November 2017


Thioredoxin-FGD fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1030
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1079
    Illegal BamHI site found at 367
    Illegal XhoI site found at 373
    Illegal XhoI site found at 1345
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 739
    Illegal NgoMIV site found at 937
    Illegal AgeI site found at 703
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Theoretically, thioredoxin (BBa_K2382004)could help protein folding in the cell, thus,we combined the FGD(BBa_K2382002) and thioredoxin together to make a new fusion protein. The two glycine between the linker and FGD are designed for a better flexibility.

Source

thioredoxin Escherichia coli str. K-12 substr. MG1655 ( NC_000913.3 )

FGD Mycobacterium smegmatis str. MC2 155 (AAC38338.1)