Difference between revisions of "Part:BBa K2442203"

Revision as of 22:15, 1 November 2017

Pmtle +RBS WT

This part contains the native pmtlE promoter and its native ribosome binding site. pmtlE is the intergenic region between mtlR and the mtlE coding region in Pseudomonas Flourescens. Naturally the pmtle promoter is regulated by the mtlR protein which complexes upon induction by sugars and activates the promoter.

The intergenic region lies between the mtlR stop codon and the mtlE start codon (158 bp). Within this intergenic region the mtle promoter resides Figure 1 illustrates where the promoter is thought to lie in the region relative to the neighboring coding sequences.

Figure 1: The DNA sequence of the intergenic region between the stop codon of mtlR and the first codon of mtlE. -10 and -35 predicted elements are shown in boxes

For this part the entire intergenic region (including the pmtle promoter) was cloned into psb1c3. Within this sequence there is a putative wild type ribosome binding site. This part was one of the variants tested with mtlR to evoke a GFP response via sugar induction.

Usage and Biology

This part was cloned into a plasmid containing GFP. The combining of these two part for the formation of our reporter plasmid and this was subsequently tested in our experimental phase.

Sequence and Features