Difference between revisions of "Part:BBa K2382012"

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===Usage and Biology===
 
===Usage and Biology===
  
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In our design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the
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two proteins from interrupting each other while folding.
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This part is a rigid linker that links two proteins to form one fusion protein. It repeats amino acids EAAAK
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A rigid linker that links two proteins to form one fusion protein. It repeats amino acids EAAAK
+
 
three times to maintain distance of two proteins and preventing from interrupting each other
 
three times to maintain distance of two proteins and preventing from interrupting each other
 
while folding.<br>
 
while folding.<br>
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The structure of this linker is basically alpha helix, indicating a rigid property. It can help the two parts in a fusion protein to maintain the distance between each other and function independently.  
+
The structure of this linker is basically alpha helix, indicating a rigid property. It can help the two parts in a fusion protein to maintain the distance between each other and function independently. <br>
 +
 
 +
In our fusion protein design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the
 +
two proteins from interrupting each other while folding. And the computer structure simulation support this description.
  
  

Latest revision as of 22:09, 1 November 2017

EAAAK


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This part is a rigid linker that links two proteins to form one fusion protein. It repeats amino acids EAAAK three times to maintain distance of two proteins and preventing from interrupting each other while folding.
The structure of this linker is basically alpha helix, indicating a rigid property. It can help the two parts in a fusion protein to maintain the distance between each other and function independently.

In our fusion protein design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the two proteins from interrupting each other while folding. And the computer structure simulation support this description.


Characterization of the EAAAK

References

Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021