Difference between revisions of "Part:BBa K2382012"

(→‎Characterization of the EAAAK)
(→‎Usage and Biology)
Line 22: Line 22:
 
A rigid linker that links two proteins to form one fusion protein. It repeats amino acids EAAAK
 
A rigid linker that links two proteins to form one fusion protein. It repeats amino acids EAAAK
 
three times to maintain distance of two proteins and preventing from interrupting each other
 
three times to maintain distance of two proteins and preventing from interrupting each other
−
while folding.
+
while folding.<br>
 +
The structure of this linker is basically alpha helix, indicating a rigid property. It can help the two parts in a fusion protein to maintain the distance between each other and function independently.
 +
 
  
  
Line 29: Line 31:
 
</html>
 
</html>
 
__TOC__
 
__TOC__
−
 
−
 
  
 
==Characterization of the EAAAK==
 
==Characterization of the EAAAK==

Revision as of 22:07, 1 November 2017

EAAAK


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

In our design, we use it as a rigid linker linking two proteins to form one fusion protein. It can prevent the two proteins from interrupting each other while folding. A rigid linker that links two proteins to form one fusion protein. It repeats amino acids EAAAK three times to maintain distance of two proteins and preventing from interrupting each other while folding.
The structure of this linker is basically alpha helix, indicating a rigid property. It can help the two parts in a fusion protein to maintain the distance between each other and function independently.


Characterization of the EAAAK

References

Yue-Jin Wu, Chia-Ying Fan, Yaw-Kuen Li. Protein purification involving a unique auto-cleavage feature of a repeated EAAAK peptide. Journal of Chromatography B, 877 (2009) 4015–4021