Difference between revisions of "Part:BBa K2205001"
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deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants. | deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants. | ||
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| + | Characterisation data for deGFP for collected using the expression construct <partinfo>BBa_K2205002</partinfo> | ||
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[[File:T--Newcastle--BB_deGFP.png|400px|]] | [[File:T--Newcastle--BB_deGFP.png|400px|]] | ||
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| + | ===FACS Data - Collected by Exeter iGEM 2017 for Newcastle iGEM 2017=== | ||
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| + | <img src="https://static.igem.org/mediawiki/2017/c/cc/T--Newcastle--BB_deGFP_FACS.png" width="600px"/> | ||
| + | Figure 5: Single cell data for '''E. coli''' cells expressing deGFP (collected by Exeter iGEM). Columns 1-6: cells expressing deGFP. Columns 7 and 8: wild type Top10 cells. Red parts of the bar are non-fluorescent cells, green parts are fluorescent cells. | ||
Revision as of 22:06, 1 November 2017
deGFP
deGFP is a variant of Green Fluorescent Protein (GFP). It was initially designed by Shin and Noireaux (2010) for expression in cell-free protein synthesis systems and is more efficiently translated than other variants.
Characterisation data for deGFP for collected using the expression construct BBa_K2205002
CFPS Expression of deGFP
CFPS Expression of J23100-deGFP and J23100-sfGFP Constructs: Time course for increase in fluorescence intensity of CFPS systems expressing GFP constructs over time. Each data point is an average of triplicate results, and error bars show +/- standard error. CFPS system with no DNA (Red) was used as the negative control.
In vivo Qualitative Comparison
FACS Data - Collected by Exeter iGEM 2017 for Newcastle iGEM 2017
<img src="
" width="600px"/>
Figure 5: Single cell data for E. coli cells expressing deGFP (collected by Exeter iGEM). Columns 1-6: cells expressing deGFP. Columns 7 and 8: wild type Top10 cells. Red parts of the bar are non-fluorescent cells, green parts are fluorescent cells.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]