Difference between revisions of "Part:BBa K2398002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This was designed for the use with the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). It is meant for the usage as building block for accessory plasmids in the context of phage assisted continuous ecolution [[#References|[1]]] or related systems [[#References|[2]]]. | + | This part was designed for the use with the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). It is meant for the usage as building block for accessory plasmids in the context of phage assisted continuous ecolution [[#References|[1]]] or related systems [[#References|[2]]]. |
The part is fully compatible with RFC10, as well as with Golden Gate assembly | The part is fully compatible with RFC10, as well as with Golden Gate assembly | ||
Revision as of 22:06, 1 November 2017
Blue light-induced promotor for application in Phage assisted continous evolution (PACE)
Design Notes
This part was designed for the use with the cloning standard of the iGEM Team Heidelberg 2017 (http://2017.igem.org/Team:Heidelberg/RFC). It is meant for the usage as building block for accessory plasmids in the context of phage assisted continuous ecolution [1] or related systems [2].
The part is fully compatible with RFC10, as well as with Golden Gate assembly
Source
Bacteriophage T7, with modifications.
References
[1] Esvelt, Kevin M.; Carlson, Jacob C.; Liu, David R. (2011): A system for the continuous directed evolution of biomolecules. In: Nature 472 (7344), S. 499–503. DOI: 10.1038/nature09929.
[2]Brödel, Andreas K.; Jaramillo, Alfonso; Isalan, Mark (2016): Engineering orthogonal dual transcription factors for multi-input synthetic promoters. In: Nature communications 7, S. 13858. DOI: 10.1038/ncomms13858.
[3] Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. 10.1016/j.cell.2013.02.022 PubMed 23452860