Difference between revisions of "Part:BBa K2442203"

Revision as of 21:59, 1 November 2017

Pmtle +RBS WT

This part contains the native pmtlE promoter and its native ribosome binding site. pmtlE is the intergenic region between mtlR and the mtlE coding region in Pseudomonas Flourescens. Naturally the pmtle promoter is regulated by the mtlR protein which complexes upon induction by sugars and activates the promoter.

The intergenic region lies between the mtlR stop codon and the mtlE start codon (158 bp). Within this intergenic region the mtle promoter resides Figure 1 illustrates where the promoter is thought to lie in the region relative to the neighboring coding sequences.

T-Glasgow-intergenicmtle.jpeg.png

For this part the entire intergenic region (including the pmtle promoter) was cloned into psb1c3. Within this sequence there is a putative wild type ribosome binding site. This part was one of the variants tested with mtlR to evoke a GFP response via sugar induction.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 The intergenic region lies between the mtlR stop codon and the mtlE start codon (158 bp). Within this intergenic region the mtle promoter resides <i>Figure 1</i> illustrates where the promoter is thought to lie in the region relative to the neighboring coding sequences.
-https://static.igem.org/mediawiki/2017/7/7b/T-Glasgow-intergenicmtle.jpeg
+https://static.igem.org/mediawiki/2017/7/7b/T-Glasgow-intergenicmtle.jpeg.png
 For this part the entire intergenic region (including the pmtle promoter) was cloned into psb1c3. Within this sequence there is a putative wild type ribosome binding site. This part was one of the variants tested with mtlR to evoke a GFP response via sugar induction.