Difference between revisions of "Part:BBa K2315024"
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==<b>Usage and Biology</b>== | ==<b>Usage and Biology</b>== | ||
| − | [[File:T--Shanghaitech-- | + | [[File:T--Shanghaitech--luxrfx.jpg|thumb|center|700px|<b>Fig. 1 Genetic Circuit Design</b>]] |
When AHL is added with concentration higher than a critical value, the constitutively expressed LuxR will bind to the AHL molecule 3OC6-HSL, dimerize and bind to the pLux regulatory sequence to activate GFP expression. | When AHL is added with concentration higher than a critical value, the constitutively expressed LuxR will bind to the AHL molecule 3OC6-HSL, dimerize and bind to the pLux regulatory sequence to activate GFP expression. | ||
Latest revision as of 21:44, 1 November 2017
The HSL receiver LuxR from Aliivibrio fischeri activates expression of GFP protein in response to 3OC6-HSL.
This Receiver can be easily replaced by other AHL receivers in our collection. A full collection could be found in: http://2017.igem.org/Team:Shanghaitech/Library
Usage and Biology
When AHL is added with concentration higher than a critical value, the constitutively expressed LuxR will bind to the AHL molecule 3OC6-HSL, dimerize and bind to the pLux regulatory sequence to activate GFP expression.