Difference between revisions of "Part:BBa K2194004:Design"
(→‎Source)
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===Source===
 
===Source===
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See the registry pages for negative feedback membrane stress promoter <i>PgntK</i> ([https://parts.igem.org/Part:BBa_K2194002  BBa_K2194002]), cysPUWA proteins ([https://parts.igem.org/Part:BBa_K2194003  BBa_K2194003]), Elowitz RBS ([https://parts.igem.org/Part:BBa_B0034  BBa_B0034]), and sulfate binding protein sbp ([https://parts.igem.org/Part:BBa_K2194001  BBa_K2194001]) sources.
  
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[update]
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The RBS before <i>cysPUWA</i> has the synthetic sequence 5’AAGCGGACGACACACAAGGCTCCACCAAA3’ and was designed with the “Salis Lab RBS Designer” [1].
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 +
The terminator “L3S3P21” after <i>sbp</i> has the synthetic sequence 5’CCAATTATTGAAGGCCTCCCTAACGGGGGGCCTTTTTTTGTTTCTGGTCTGCC3’. It is sourced from Chen et al [2] and is notable because it is a strong, recombination-resistant terminator.
  
 
===References===
 
===References===
  
 
[update]
 
[update]

Revision as of 21:43, 1 November 2017


Sulfate Transport System w/ Negative Feedback for Membrane Stress


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2296
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[update]

Source

See the registry pages for negative feedback membrane stress promoter PgntK (BBa_K2194002), cysPUWA proteins (BBa_K2194003), Elowitz RBS (BBa_B0034), and sulfate binding protein sbp (BBa_K2194001) sources.

The RBS before cysPUWA has the synthetic sequence 5’AAGCGGACGACACACAAGGCTCCACCAAA3’ and was designed with the “Salis Lab RBS Designer” [1].

The terminator “L3S3P21” after sbp has the synthetic sequence 5’CCAATTATTGAAGGCCTCCCTAACGGGGGGCCTTTTTTTGTTTCTGGTCTGCC3’. It is sourced from Chen et al [2] and is notable because it is a strong, recombination-resistant terminator.

References

[update]