Difference between revisions of "Part:BBa K2136013"

Latest revision as of 21:40, 1 November 2017

hsp70 + rbsc2 synthetic constitutive promoter for Chlamydomonas

AR1-Rbcs2

Constitutive promoter for use in Chlamydomonas reinhardtii based on the fusion of DNA sequences upstream of Heat Shock Protein 70A (nucleotides 1-275) and rbcS2 (nucleotides 294-474) genes. This part also includes a 5'-UTR derived from the rbcS2 gene.

Improvement

NU_Kazakhstan 2017 team made an improved version of this promoter by inserting high-efficiency separator between two promoters and added the first intron of RBCS2 gene. Link: BBa_K2516001

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 264
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 18:21, 1 November 2017 (view source) Valdemirkim (Talk \| contribs) ← Older edit		Latest revision as of 21:40, 1 November 2017 (view source) Valdemirkim (Talk \| contribs)
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−	~~<b>Improved part in 2017</b>~~	+	===Improvement===

−	NU_Kazakhstan 2017 team made an improved version of this promoter by inserting high-efficiency separator between two promoters and added the first intron of RBCS2 gene. Link: https://parts.igem.org/Part:BBa_K2516001	+	NU_Kazakhstan 2017 team made an improved version of this promoter by inserting high-efficiency separator between two promoters and added the first intron of RBCS2 gene. Link: [https://parts.igem.org/Part:BBa_K2516001 BBa_K2516001]