Difference between revisions of "Part:BBa K2515003:Experience"
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===Applications of BBa_K2515003=== | ===Applications of BBa_K2515003=== | ||
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| + | We used this part instead of BBa_K145201 for fusions with GFR generator (BBa_K081012) and RFP generator (BBa_K081014). It functioned as expected obtaining lower basal level of expression in the high copy number vector pSB1C3.<br /><br /> | ||
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| + | We believe that the major reason for the improvement is the removal of the LVA-like tag from TetR and the inverted orientation is not so critical but this hypothesis needs further experiments to be proven. | ||
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| + | We also cloned this part together with a reversed transcriptional terminator (Part:BBa_B0025). We did not observe a functional improvement. | ||
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===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 21:34, 1 November 2017
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2515003
We used this part instead of BBa_K145201 for fusions with GFR generator (BBa_K081012) and RFP generator (BBa_K081014). It functioned as expected obtaining lower basal level of expression in the high copy number vector pSB1C3.
We believe that the major reason for the improvement is the removal of the LVA-like tag from TetR and the inverted orientation is not so critical but this hypothesis needs further experiments to be proven.
We also cloned this part together with a reversed transcriptional terminator (Part:BBa_B0025). We did not observe a functional improvement.
User Reviews
UNIQ7b215dbe1c7cc619-partinfo-00000000-QINU UNIQ7b215dbe1c7cc619-partinfo-00000001-QINU