Difference between revisions of "Part:BBa K2195000:Design"
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | This comes from the genomic sequence of the Tardigrade Species Ramazzottius varieornatus | + | This comes from the genomic sequence of the Tardigrade Species Ramazzottius varieornatus. The gene was discovered by Hashimoto et al. (1) |
===References=== | ===References=== | ||
| + | 1. Hashimoto, T. et al. Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nature Communications 7, 12808, doi: 10.1038/ncomms12808 (2016). | ||
Latest revision as of 21:34, 1 November 2017
Dsup (Radiation Protection)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
As this protein came from a eukaryotic species, it was not codon optimized for E. Coli. In addition, there were several illegal restriction sites that had to be replaced with different codons.
Source
This comes from the genomic sequence of the Tardigrade Species Ramazzottius varieornatus. The gene was discovered by Hashimoto et al. (1)
References
1. Hashimoto, T. et al. Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nature Communications 7, 12808, doi: 10.1038/ncomms12808 (2016).