Difference between revisions of "Part:BBa K2378004"

Revision as of 21:22, 1 November 2017

pSal + PETase Coding Device

ITB_Indonesia iGEM team 2017 designed a PETase enzyme coding device consisting of NahR-pSal regulatory part (BBa_J61051, https://parts.igem.org/Part:BBa_J61051) which is commonly activated by salicylate induction. Besides salicylate, NahR-pSal regulatory system was also known to have a certain degree of responsiveness in the presence of organic pollutants (OPs). This particular characteristic of NahR-pSal is utilized in iGEM 2017 Indonesia's project to detect the presence of plastic.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 1303
Illegal NotI site found at 2212
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 786
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1957
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2378004 short</partinfo>
-ITB_Indonesia iGEM team 2017 designed a PETase enzyme coding device consisting of NahR-pSal regulatory part (BBa_J61051, https://parts.igem.org/Part:BBa_J61051) which is activated by salicylate inducer. In effect, PETase is only expressed when this device detect the presence of salicylate, which binds to NahR.
+ITB_Indonesia iGEM team 2017 designed a PETase enzyme coding device consisting of NahR-pSal regulatory part (BBa_J61051, https://parts.igem.org/Part:BBa_J61051) which is commonly activated by salicylate induction. Besides salicylate, NahR-pSal regulatory system was also known to have a certain degree of responsiveness in the presence of organic pollutants (OPs). This particular characteristic of NahR-pSal is utilized in iGEM 2017 Indonesia's project to detect the presence of plastic.
 <!-- Add more about the biology of this part here