Difference between revisions of "Part:BBa K2384007:Design"

 
Line 14: Line 14:
  
 
Our sequence optimization is based on the sequence provided by Cornell University's IGEM VIKI in 2014.
 
Our sequence optimization is based on the sequence provided by Cornell University's IGEM VIKI in 2014.
 
+
This gene is composed of synthetic biology company.
 
===References===
 
===References===

Latest revision as of 21:16, 1 November 2017


GST-CRS5


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 673
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85


Design Notes

It is more suitable for expression in Bacillus megaterium.And there is no termination codon in this CDS.And this CDS is with a T7 terminator.


Source

Our sequence optimization is based on the sequence provided by Cornell University's IGEM VIKI in 2014. This gene is composed of synthetic biology company.

References