Difference between revisions of "Part:BBa K2524001"
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<span class='h3bb'>Sequence and Features</span>
 
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<td width="50%" align=center><p align=left><b>A</b><br></p>
 
http://2017.igem.org/File:T--Georgia_State--factorc3D.jpg
 
http://2017.igem.org/File:T--Georgia_State--factorc3D.jpg
  

Revision as of 21:13, 1 November 2017


Limulus Clotting Factor C

Factor C is an intracellular endotoxin sensitive serine protease zymogen which initiates a coagulation cascade in the hemolymph of the Horseshoe crab (Limulus). It is apart of their immune system. The Factor C protein is the first in a cascade that causes their blood to clot in the presence of lipopolysaccharide (LPS) located on the outer membrane of gram-negative bacteria. When Factor C is in the presence of LPS it undergoes autocatalysis. This is very helpful upon detection of minuscule concentrations of gram-negative bacteria. Factor C is a glycoprotein with a molecular mass of 123 kD. It has an Heavy chain (80kD) and Light chain (43 kD). In the presence of LPS, factor C is activated and undergoes autocatalysis and cleaves the Phe-Ile bond. This autocatalysis results in the formation of two new fragments: B chain (34kD) and A chain (8.5 kD). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

A

http://2017.igem.org/File:T--Georgia_State--factorc3D.jpg