Difference between revisions of "Part:BBa K2515003:Design"

(→‎Design Notes)
(→‎Design Notes)
 
Line 9: Line 9:
  
 
<br /><br />
 
<br /><br />
 +
<p align="justify">
 
This part was PCR amplified using BBa_K145201 as matrix and then cloned in pSB1C3.
 
This part was PCR amplified using BBa_K145201 as matrix and then cloned in pSB1C3.
 
<br /><br />
 
<br /><br />
Line 19: Line 20:
 
The resulting product should be purified, digested with EcoRI and PstI and ligated in pSB vector of choice.
 
The resulting product should be purified, digested with EcoRI and PstI and ligated in pSB vector of choice.
 
<br /><br />
 
<br /><br />
−
<b>Important:</b> In contrast to the original part, this one has TetR with no LVA-like tag and no transcriptional terminators. If you want to add terminator, we do recommend part Part:BBa_B0025.
+
<b>Important:</b> In contrast to the original part, this one has TetR with no LVA-like tag and no transcriptional terminators. <br />If you want to add terminator, we do recommend part Part:BBa_B0025.</p>
  
 
===Source===
 
===Source===

Latest revision as of 21:10, 1 November 2017


Inverted INPUT TetR generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes



This part was PCR amplified using BBa_K145201 as matrix and then cloned in pSB1C3.

To clone this part by yourself, use the following primer pair:

Tet_Gen_InvF TATCTGCAGCGGCCGCTACTAGTAttgacagctagctcagtcc
Tet_Gen_InvR TATGAATTCGCGGCCGCTTCTAGAGTTAcccactttcacatttaagttg

The resulting product should be purified, digested with EcoRI and PstI and ligated in pSB vector of choice.

Important: In contrast to the original part, this one has TetR with no LVA-like tag and no transcriptional terminators.
If you want to add terminator, we do recommend part Part:BBa_B0025.

Source

Part:BBa_K145201

References