Difference between revisions of "Part:BBa K2446021:Design"

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===Design Notes===
 
===Design Notes===
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This part is aseembled by the specfic DNA binding sites and Sv40 promoter through Biobrick assembly. We inset the DNA binding sites behind the Sv40 promoter. Then the DNA binding domain with KRAB can repress the expression of this promoter. The repression intensity can be adjusted by change the repeats of DNA binding sites and build logical gate.
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This part is aseembled by the specfic DNA binding sites and minCMV promoter through Biobrick assembly. We inset the DNA binding sites before the minCMV promoter. Then the DNA binding domain with VP64 can active the expression of this promoter. The activation intensity can be adjusted by change the repeats of DNA binding sites and build logical gate.
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===Source===
 
===Source===

Latest revision as of 21:10, 1 November 2017


CMV_8_ZF54-8/43-8 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is aseembled by the specfic DNA binding sites and minCMV promoter through Biobrick assembly. We inset the DNA binding sites before the minCMV promoter. Then the DNA binding domain with VP64 can active the expression of this promoter. The activation intensity can be adjusted by change the repeats of DNA binding sites and build logical gate.

Source

Assembled by BBa_K2446053 (minCMV) and BBa_K2446016 (8_ZF_54-8/43-8 binding site) through Biobrick assembly.

References

[1] R. R. Beerli, D. J. Segal, B. Dreier, C. F. Barbas, Toward controlling gene expression at will: specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proceedings of the National Academy of Sciences 95, 14628-14633 (1998).