Difference between revisions of "Part:BBa K2457006"
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===Characterization===
 
===Characterization===
  
 +
===Sequencing===
 
[[File:BBa K2457006 electropherogram.png]]
 
[[File:BBa K2457006 electropherogram.png]]
  

Revision as of 21:03, 1 November 2017


Cas9 device + Optimized sgRNA

descrever

Usage and Biology

Design

BBa K2457006 circuit.png

Figure 1: BBa K2457006 circuit

Characterization

Sequencing

BBa K2457006 electropherogram.png

Figure 2:Sequencing electropherogram from BBa_K2457006.

BBa K2457006 alignment.png

Figure 3:Alignment of the designed sequence and our final construction from BBa_K2457006.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1207
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1024