Difference between revisions of "Part:BBa K2446021:Design"
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===Source=== | ===Source=== | ||
| − | Assembled by | + | Assembled by BBa_K2446053 (minCMV) and BBa_K2446016 (8_ZF_54-8/43-8 binding site) through Biobrick assembly. |
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===References=== | ===References=== | ||
[1] R. R. Beerli, D. J. Segal, B. Dreier, C. F. Barbas, Toward controlling gene expression at will: specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proceedings of the National Academy of Sciences 95, 14628-14633 (1998). | [1] R. R. Beerli, D. J. Segal, B. Dreier, C. F. Barbas, Toward controlling gene expression at will: specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proceedings of the National Academy of Sciences 95, 14628-14633 (1998). | ||
Revision as of 21:02, 1 November 2017
CMV_8_ZF54-8/43-8 promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is aseembled by the specfic DNA binding sites and Sv40 promoter through Biobrick assembly. We inset the DNA binding sites behind the Sv40 promoter. Then the DNA binding domain with KRAB can repress the expression of this promoter. The repression intensity can be adjusted by change the repeats of DNA binding sites and build logical gate.
Source
Assembled by BBa_K2446053 (minCMV) and BBa_K2446016 (8_ZF_54-8/43-8 binding site) through Biobrick assembly.
References
[1] R. R. Beerli, D. J. Segal, B. Dreier, C. F. Barbas, Toward controlling gene expression at will: specific regulation of the erbB-2/HER-2 promoter by using polydactyl zinc finger proteins constructed from modular building blocks. Proceedings of the National Academy of Sciences 95, 14628-14633 (1998).