Difference between revisions of "Part:BBa K2271142"
Line 6: Line 6:
 
Pex5 is one of two peroxins that mediate protein import into the peroxisomal matrix. Proteins, destined for the peroxisome, are tagged with so called peroxisomal targeting signals. Whilst Pex5 detects the Pts1, Pex 7 detects the Pts2.
 
Pex5 is one of two peroxins that mediate protein import into the peroxisomal matrix. Proteins, destined for the peroxisome, are tagged with so called peroxisomal targeting signals. Whilst Pex5 detects the Pts1, Pex 7 detects the Pts2.
 
<br>
 
<br>
−
We designed a wild type Pts1 to tag proteins of interest for peroxisomal translocation. This Pts1 sequence was taken from <a href="https://www.ncbi.nlm.nih.gov/pubmed/27025684"><abbr="Towards repurposing the yeast peroxisome
+
This part is a wild type Pts1 sequence for tagging proteins for peroxisomal translocation.
−
for compartmentalizing heterologous metabolic
+
−
pathways">Dueber <i>et al.</i> (2016)</abbr></a>
+
 
</p></html>
 
</p></html>
  

Revision as of 21:01, 1 November 2017


Wild type Pts1

Pex5 is one of two peroxins that mediate protein import into the peroxisomal matrix. Proteins, destined for the peroxisome, are tagged with so called peroxisomal targeting signals. Whilst Pex5 detects the Pts1, Pex 7 detects the Pts2.
This part is a wild type Pts1 sequence for tagging proteins for peroxisomal translocation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 34
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]