Difference between revisions of "Part:BBa K2271142"
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Pex5 is one of two peroxins that mediate protein import into the peroxisomal matrix. Proteins, destined for the peroxisome, are tagged with so called peroxisomal targeting signals. Whilst Pex5 detects the Pts1, Pex 7 detects the Pts2.
 
Pex5 is one of two peroxins that mediate protein import into the peroxisomal matrix. Proteins, destined for the peroxisome, are tagged with so called peroxisomal targeting signals. Whilst Pex5 detects the Pts1, Pex 7 detects the Pts2.
 
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In the process of our project we came up with several Pts1 variant that were supposed to function in combination with designed Pex5 variants. This part is one of the Pts1 variants we came up with &minus; it is the result of molecular dynamics simulations. We call it P3.  
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We designed a wild type Pts1 to tag proteins of interest for peroxisomal translocation. This Pts1 sequence was taken from <a href="https://www.ncbi.nlm.nih.gov/pubmed/27025684"><abbr="Towards repurposing the yeast peroxisome
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for compartmentalizing heterologous metabolic
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pathways">Dueber <i>et al.</i> (2016)</abbr></a>
 
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Revision as of 21:00, 1 November 2017


Wild type Pts1

Pex5 is one of two peroxins that mediate protein import into the peroxisomal matrix. Proteins, destined for the peroxisome, are tagged with so called peroxisomal targeting signals. Whilst Pex5 detects the Pts1, Pex 7 detects the Pts2.
We designed a wild type Pts1 to tag proteins of interest for peroxisomal translocation. This Pts1 sequence was taken from Dueber et al. (2016)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 34
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]