Difference between revisions of "Part:BBa K2324015"

Revision as of 20:58, 1 November 2017

Arabinose inducible fim operon

description of the arabinoise inducible fim operon.

This part contains the fim operon under the control of an inducible arabinose promoter. The operon contains six Fim proteins, including FimA, FimI, FimC, FimD, FimF and FimG. When co-transformed with FimH constructs from our project, which have rhamnose-inducible promoters, modified type I pili may be produced. The production of the operon must be initiated by inducing the culture overnight at 0.6 OD at 2% arabinose.

Figure 1 We transformed a plasmid containing the fim operon under the control of the P_Ara promoter. It exhibits flagella on their cell surface, but the electron micrograph shows a suggestion of pili.

This suggests that the P_Ara construct was successful in synthesising pili (minus FimH)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
Illegal BamHI site found at 2743
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1026
Illegal AgeI site found at 1057
1000
COMPATIBLE WITH RFC[1000]

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 <img src="https://static.igem.org/mediawiki/2017/1/17/T--Exeter--FimBKOPARApili.jpeg" height="475px" width="750px"/>
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-<b>Figure 1</b> We transformed a plasmid containing the <i>fim operon</i> under the control of the P_Ara promoter. It exhibits flagella on their cell surface, but the electron micrograph shows a suggestion of pili.
+<b>Figure 1</b>
+We transformed a plasmid containing the <i>fim operon</i> under the control of the P_Ara promoter. It exhibits flagella on their cell surface, but the electron micrograph shows a suggestion of pili.
 This suggests that the P_Ara construct was successful in synthesising pili (minus FimH)