Difference between revisions of "Part:BBa K2443034"

Latest revision as of 20:54, 1 November 2017

CK optimized for expression in E. coli

Creatine kinase (CK) is responsible for catalyzing the conversion of creatine. It has been Codon optimized for use in Escherichia coli and contains a C-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator. Overexpression of CK was performed from BL21DE3 Gold including pJET with the K2443034 coding sequence inserted. The expected size of CK is 44 kDa, which corresponds to the band observed in lane 2 on the overexpression gel below. Overexpressions were performed according to the protocol and notebook on the iGEM 2017 wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 914
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2443034 short</partinfo>
-Creatine kinase (CK) is responsible for catalyzing the conversion of creatine. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a C-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator.
+Creatine kinase (CK) is responsible for catalyzing the conversion of creatine. It has been Codon optimized for use in <i>Escherichia coli</i> and contains a C-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator. Overexpression of CK was performed from BL21DE3 Gold including pJET with the K2443034 coding sequence inserted. The expected size of CK is 44 kDa, which corresponds to the band observed in lane 2 on the overexpression gel below. Overexpressions were performed according to the protocol and notebook on the iGEM 2017 wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).
+https://static.igem.org/mediawiki/parts/0/06/Lethbridge_oct_5_gel_6.png
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