Difference between revisions of "Part:BBa K2443033"

myokinase (MK) is a phosphotransferase enzyme that is responsible for the catalyzing the interconversion of adenine nucleotides (ATP, ADP, and AMP). By constantly monitoring phosphate nucleotide levels inside the cell, ADK plays an important role in cellular energy homeostasis. MK has been Codon optimized for use in <i>Escherichia coli</i> and contains a N-terminal hexahistidine tag. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of MK is 25 kDa, and K2443033 expression is seen in lanes 4 and 5. Protein overexpression was performed in BL21DE3 gold containing pUC57-kan with K2443033 coding sequence inserted. Proteins were expressed according to the overexpression protocol and notebook on the 2017 iGEM lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).