Difference between revisions of "Part:BBa K2378007"

Revision as of 20:46, 1 November 2017

IrrE Coding Sequence

This is improved coding sequence of IrrE from BBa_K729001 (https://parts.igem.org/Part:BBa_K729001). When transformed to E. coli, this protein protected from salt, oxidative, and thermal shock.

Background of Improvement

We tried to analyse existing IrrE part from BBa_K729001 Team UCL London, and found some possible error in the coding sequence. The translated nucleotide is not a contiguous protein, which is shown by a stop codon in the middle of the coding sequence, continued by another start codon ~90 base downstream.

At first, we thought that it's the IrrE nature to have two separate active proteins, but after we find the Uniprot accession number Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 489
Illegal AgeI site found at 606
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This is improved coding sequence of IrrE from BBa_K729001 (https://parts.igem.org/Part:BBa_K729001). When transformed to E. coli, this protein protected from salt, oxidative, and thermal shock.
+<i>Background of Improvement</i>
+We tried to analyse existing IrrE part from BBa_K729001 Team UCL London, and found some possible error in the coding sequence. The translated nucleotide is not a contiguous protein, which is shown by a stop codon in the middle of the coding sequence, continued by another start codon ~90 base downstream.
+At first, we thought that it's the IrrE nature to have two separate active proteins, but after we find the Uniprot accession number
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+===Background of Improvement===
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