Difference between revisions of "Part:BBa K2271006"
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===Usage and Biology=== | ===Usage and Biology=== | ||
Pex34p is a peroxisomal integral membrane protein that can act both, independently and in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division. | Pex34p is a peroxisomal integral membrane protein that can act both, independently and in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 20:25, 1 November 2017
Pex34p-mTurquoise with ScCCW12 promoter
This part contains the coding sequence for the peroxin Pex34p with a strong constitive promoter ScSSW12 and the terminator ScADH1. It is codon-optimized for S. cerevisiae and contains the fluorescent protein mTurquoise fused to the 3'-end of the coding sequence. This is used in order to visualize the peroxin in the cell.
Usage and Biology
Pex34p is a peroxisomal integral membrane protein that can act both, independently and in combination with Pex11p, Pex25p, and Pex27p to control the peroxisome morphology and population. Pex34p is suggested to directly influence peroxisome proliferation as well as constitutive peroxisome division.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 701
Illegal BamHI site found at 1139
Illegal XhoI site found at 1862 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]