Latest revision as of 20:18, 1 November 2017

BXB1 att B

The attP site is the phage attachment site for the Bxb1 phage. The site is recognized by Bxb1 integrase. The Bxb1 integrase catalyzes a unidirectional strand exchange between a 53 bp attP site and a 50 bp attB site (Bonnet et al., 2012). The enzyme works by making a synapse between attP and attB, making double strand breaks producing a 2 bp sticky overhang, exchanging the strands, and re-ligating them in the recombinant configuration (attL and attR).

Reference: Jerome Bonnet, Pakpoom Subsoontorn, and Drew Endy. Rewritable digital data storage in live cells via engineered control of recombination directionality. PNAS 2012 ; published ahead of print May 21, 2012, doi:10.1073/pnas.1202344109

Characterization by Fudan_China 2017

• Group: Fudan_China 2017
• Author: Haiyun Liu, Fudan University

We characterized the kinetic characterization and the orthogonality between Bxb1 and some other serine recombinase using this attB site. For more information, go to Mycobacterium Phage Bxb1 gp35, DNA integrase

Sequence and Features