Difference between revisions of "Part:BBa K2443022"

Latest revision as of 20:12, 1 November 2017

MTF optimized for expression in E. coli

Methionyl-tRNA transformyalse (MTF) is a zinc finger metal response element-binding transcription factor. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag. The expected size of MTF is 36 kDa, which closely corresponds to the bands seen the induced lane (lane 1) of the overexpression gel below. Protein overexpression was performed from BL21DE3 gold containing pJET vector with BBa K2443022 insert. Protein overexpression was performed according to the protocol, and notebook on the 2017 iGEM lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 888
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 244
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2443022 short</partinfo>
-Methionyl-tRNA transformyalse (MTF) is a zinc finger metal response element-binding transcription factor. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag. The expected size of MTF is 36 kDa, which closely corresponds to the bands seen the induced lane of the overexpression gel below. Protein overexpression was performed from BL21DE3 gold containing pJET vector with BBa K2443022 insert. Protein overexpression was performed according to the protocol, and notebook on the 2017 iGEM lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).
+Methionyl-tRNA transformyalse (MTF) is a zinc finger metal response element-binding transcription factor. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag. The expected size of MTF is 36 kDa, which closely corresponds to the bands seen the induced lane (lane 1) of the overexpression gel below. Protein overexpression was performed from BL21DE3 gold containing pJET vector with BBa K2443022 insert. Protein overexpression was performed according to the protocol, and notebook on the 2017 iGEM lethbridge wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).
+https://static.igem.org/mediawiki/parts/8/89/Lethbridge_oct_12_gel_1_v2.png
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