Difference between revisions of "Part:BBa K2324006"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2324006 short</partinfo> | <partinfo>BBa_K2324006 short</partinfo> | ||
| − | + | <p> | |
| + | The literature has shown that the terminal pili protein FimH (Le Trong et al 2010) can be modified by inserting heterologous sequences at position 225 and 258 (Pallesen et al 1995, Shembri et al 1999). However these two amino acids are in the pilin binding domain which may present difficulties when attempting to introduce large modifications. Harvard iGEM 2015 also introduced modifications at position 225 of the mature FimH protein.</p> | ||
| + | <p> | ||
This part produces a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence | This part produces a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence | ||
is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, | is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, | ||
produces a fluorescent FimH protein that should initiate pilus biosynthesis when co-transformed with a plasmid | produces a fluorescent FimH protein that should initiate pilus biosynthesis when co-transformed with a plasmid | ||
| − | containing the <i> fim operon </i>. | + | containing the <i> fim operon </i>.</p> |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 20:03, 1 November 2017
pRha_FimH_225sfGFP
The literature has shown that the terminal pili protein FimH (Le Trong et al 2010) can be modified by inserting heterologous sequences at position 225 and 258 (Pallesen et al 1995, Shembri et al 1999). However these two amino acids are in the pilin binding domain which may present difficulties when attempting to introduce large modifications. Harvard iGEM 2015 also introduced modifications at position 225 of the mature FimH protein.
This part produces a FimH adhesin protein fused with sfGFP at its 225th amino acid residue. The coding sequence is under the control of a rhamnose-inducible promoter, with a B0034 RBS and a B0015 terminator. The part, when induced, produces a fluorescent FimH protein that should initiate pilus biosynthesis when co-transformed with a plasmid containing the fim operon .
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 451
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]