Difference between revisions of "Part:BBa K2443017"

Latest revision as of 19:59, 1 November 2017

SerRS optimized for expression in E. coli

Serine tRNA synthetase responsible for attaching serine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size for SerRS was 50 kDa, which corresponds to the band observed in lane 7 of the overexpression gel below. Protein was expressed from BL21DE3 containing pUC57-kan plasmid and the coding sequence for K2443017. Overexpressions were performed according to the protocols, and notebook on the 2017 iGEM Lethbridge team page (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 149

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 <partinfo>BBa_K2443017 short</partinfo>
-Serine tRNA synthetase responsible for attaching serine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.
+Serine tRNA synthetase responsible for attaching serine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size for SerRS was 50 kDa, which corresponds to the band observed in lane 7 of the overexpression gel below. Protein was expressed from BL21DE3 containing pUC57-kan plasmid and the coding sequence for K2443017. Overexpressions were performed according to the protocols, and notebook on the 2017 iGEM Lethbridge team page (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).
+https://static.igem.org/mediawiki/parts/d/d8/Lethbridge_Oct_17_gel_2_v2.png