Difference between revisions of "Part:BBa K2443014"
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<partinfo>BBa_K2443014 short</partinfo> | <partinfo>BBa_K2443014 short</partinfo> | ||
| − | phenylalanine tRNA synthetase responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. | + | phenylalanine tRNA synthetase responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of PheRS alpha subunit is 38.1 kDa, and corresponds to the band shown in lane 3 at that size. Protein was overexpressed from BL21DE3 gold containing a pUC57-kan vector including the coding sequence for K2443014. Protein overexpressions were done according to the protocols, and notebook on the iGEM 2017 wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf). |
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| + | https://static.igem.org/mediawiki/parts/d/d8/Lethbridge_Oct_17_gel_2_v2.png | ||
Latest revision as of 19:55, 1 November 2017
PheRS Alpha subunit optimized for expression in E. coli
phenylalanine tRNA synthetase responsible for attaching phenylalanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal hexahistidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator. The expected size of PheRS alpha subunit is 38.1 kDa, and corresponds to the band shown in lane 3 at that size. Protein was overexpressed from BL21DE3 gold containing a pUC57-kan vector including the coding sequence for K2443014. Protein overexpressions were done according to the protocols, and notebook on the iGEM 2017 wiki (https://static.igem.org/mediawiki/2017/2/2c/Protein_Overexpression_.pdf and https://static.igem.org/mediawiki/2017/0/0d/October_.pdf).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]